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KMID : 0380620080400060674
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 6 p.674 ~ p.679
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Effects of Scutellaria radix Extract on Osteoblast Differentiation and Osteoclast Formation
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Shin Jeong-Min
Park Chan-Kyung
Shin Eun-Ju
Jo Tae-Hyung
Hwang In-Kyeong
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Abstract
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Scutellaria radix (SR) has been utilized as a traditional medicine for a variety of diseases including Rheumatoidarthritis and its major flavonoids-baicalein, baicalin, and wogonin-have been reported to exert beneficial health effects,including anti-bacterial, anti-viral, anti-inflammatory, and free-radical scavenging. However, the mechanisms underlying thiseffect remain poorly understood. The principal objective of this study was to determine the effect of SR on osteoblast andosteoclast cells. SR extract was prepared using 70% ethanol solvent. Osteoblastic MC3T3-E1 cells and osteoclast precursorRaw 264.7 macrophage cells were utilized. SR extract increased MC3T3-E1 cell proliferation and stimulated alkalinephosphatase activity dose-dependently, 152.0% of the control at concentration 1μg/mL. Additionally, SR extract (1μg/mL)stimulated Bone nodule formation activity in MC3T3-E1 cells, approximately 223.3% of the control, 20 days after theexposure. In addition, SR extract significantly reduced the number of tartrate-resistant acid phosphatase-positive (TRAP+)multinucleated cells from Raw 264.7 cells. In conclusion, SR extract stimulates the proliferation and bioactivities of bone-forming osteoblasts, and inhibits the activities of bone-resorbing osteoclasts to a certain degree.
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KEYWORD
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Scutellaria radix, osteoblast, osteoclast, alkaline phosphatase, tartrate-resistant acid phosphatase
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